Random mutagenesis of BoNT/E Hc nanobody to construct a secondary phage-display library
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چکیده
منابع مشابه
Puzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
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Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies,...
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BACKGROUND Nanobodies (Nbs) are single-domain antigen-binding fragments derived from the camelids heavy-chain only antibodies (HCAbs). Their unique advantageous properties make Nbs highly attractive in various applications. The general approach to obtain Nbs is to isolate them from immune libraries by phage display technology. However, it is unfeasible when the antigens are toxic, lethal, trans...
متن کاملImproving the Specificity of an Anti-Estradiol Antibody by Random Mutagenesis and Phage Display
Estradiol is a steroid hormone secreted by endocrine glands (ovary, testis) [3]. The variation of estradiol levels in blood or urine may be associated with many pathologies such as hormone-dependent cancers and diseases of sexual behaviour [5]. Measurements of the concentration of estradiol in blood or urine is of great importance both for clinical diagnosis and during therapy. Competitive immu...
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We have isolated anti-glutathione antibodies from a human synthetic phage antibody scFv library (Nissim,A., Hoogenboom,H.R., Tomlinson,I.M., Flynn,G., Midgley,C., Lane,D. and Winter,G., 1994, EMBO J., 13, 692-698). Glutathione (GSH) conjugates with carrier proteins, such as bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH) and human lysozyme (LZM), were used as antigens. After four cy...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2014
ISSN: 1364-5072
DOI: 10.1111/jam.12526